Coding
IbpB

Part:BBa_K1620003:Design

Designed by: Celio Dias Santos Jr   Group: iGEM15_UFSCar-Brasil   (2015-09-03)


small heat shock protein IbpB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was made using original codon frequencies of E. coli.



Source

This part was designed from gene IbpB of E. coli K12 MG1655 genome, and synthesized by IDT. PCR amplifications were carried out using primers showed bellow and the 3A assembly method was made.

PCR primers:

Fwd: 5' - GAA TTC GCG GCC GCT TCT AGA GAT GAC TAT GCG TAA CTT CGA - 3'

Rev: 5' - TAC TAG TAG CGG CCG CTG CAG TTA GCT ATT TAA CGC GGG AC - 3'

References